FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004920901

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920901
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5054075
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT284530.5629714636209395No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164680.3258360827648976No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA142010.28098118844694625No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139000.2750255981559435No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC93120.1842473647502263No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC78820.15595336436439902No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC77150.15264909998367654No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC75710.14979991393083797No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA72420.14329031524067212No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC71620.14170743410020628No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT71040.14055984527336854No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA233150.071.169611
GGTATCA100350.067.633721
TATCAAC351900.047.719812
ATCAACG370900.045.419473
CAACGCA379100.044.405695
AACGCAG383700.044.150786
TCAACGC384400.043.8398134
ACGCAGA403800.041.761787
CGCAGAG412050.040.8967938
GCAGAGT430200.039.240439
AGAGTAC423000.033.95932410-11
GGGCTAT67100.028.071766
CAGAGTA430300.027.7974789
AGTACGG329050.027.73717712-13
GCTATTG70350.027.112778
ATTTAGG72500.026.5596031
GGCTATT72200.026.500357
AGTACTT199750.026.16266612-13
CTATTGA74250.026.0087729
AGGGCTA74150.025.9644835
Produced by FastQC (version 0.11.5)