FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004920907

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920907
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6103619
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT206680.33861877682732167No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT109910.18007349410243334No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT91680.15020596796752878No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA89560.14673261879550478No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG84810.13895035060346983No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT83100.13614873405433728No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT70670.11578376697497009No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC5350.064.845541
CGGGACA4100.063.2172974
CTCGCTC5600.057.8541683
CGGGGAC3050.054.293184
TCGCTCT7400.049.6198844
GTACGGG84800.049.2634351
CGCTCTG7650.048.9398655
GGGGTTA70900.047.321726
GATAGCG3200.047.2495042
TACGGGA40550.046.3423042
GAGTACG58850.044.8269421
TAGGGGT71300.044.6323784
AGGGGTT73300.043.7096025
GGGTTAG80100.042.785377
ATAGGGG77750.042.595883
ACGGGGA15350.040.8057173
AGTACGG64600.040.5692372
TACGGGG34250.039.7309722
TCTCGCT9450.039.6186332
TCTGTCG11700.038.7675558
Produced by FastQC (version 0.11.5)