FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004920913

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920913
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6220551
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT417490.6711463341430687No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208890.33580626539353187No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192790.3099243137786347No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA332900.072.292091
GGTATCA132600.072.1940841
ATCAACG499050.048.3630873
CAACGCA500950.048.191135
TCAACGC501900.048.1121374
TATCAAC504850.047.9486962
AACGCAG512650.047.109926
ACGCAGA541850.044.5602237
CGCAGAG558350.043.2533578
GCAGAGT589600.041.0414549
AGAGTAC571900.034.9515310-11
AGTACGG421850.030.41311612-13
CAGAGTA577750.029.25039710-11
AGTACTT249650.028.66427812-13
TACGGGT82000.026.14604614-15
GAGTACT246750.025.44975512-13
TACGGGC119350.025.13930714-15
GAGTACG425250.024.9937712-13
TACGGGG136150.024.50528714-15
TACGGGA167800.024.11852614-15
Produced by FastQC (version 0.11.5)