FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004920914

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920914
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6220551
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN62850.10103606577616676No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA252750.069.6162341
GGTATCA107800.060.6085551
CAACGCA351900.049.1806535
TCAACGC353600.049.0346574
ATCAACG356500.048.720413
AACGCAG360150.048.209746
TATCAAC369300.047.370112
ACGCAGA369400.047.0216677
CGCAGAG387600.044.827998
GCAGAGT407000.042.696049
AGAGTAC388350.034.8931210-11
AGTACGG446350.030.13140512-13
CAGAGTA398850.029.94112810-11
GAGTACG449800.026.40983412-13
TACGGGG145450.025.18939814-15
TACGGGT86400.025.1263814-15
GTACGGG472650.024.5572514-15
TACGGGC129550.024.42446314-15
TACGGGA173950.024.15675414-15
ACGGGAT40200.019.7562316-17
Produced by FastQC (version 0.11.5)