FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004920921

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920921
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4645305
Sequences flagged as poor quality0
Sequence length125
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290850.6261160462014873No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74900.16123806725284992No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73590.15841801560930877No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA221950.077.433881
GCTATCA37150.058.978041
CGTACGG16550.058.9147032
TATCAAC300100.057.1383672
ATCAACG316950.056.787473
GCGTACG17950.056.504961
TCAACGC328800.055.1145944
CAACGCA334600.054.3267025
AACGCAG339850.053.5204356
GGTATCA71650.050.757581
ACGCAGA359600.050.440747
CGCAGAG370000.049.113818
GCAGAGT387400.046.7198379
CTATCAA46300.046.5963752
AGAGTAC377950.031.95003510-11
CAGAGTA385050.031.09895310-11
GAGTACT169550.027.82740212-13
AGTACGG271800.026.8732412-13
AGTACTT178000.026.38046512-13
GAGTACG276550.026.12799512-13
Produced by FastQC (version 0.11.5)