FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004920945

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920945
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3581583
Sequences flagged as poor quality0
Sequence length150
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT165510.4621140987099839No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA99470.2777263573118367No Hit
GCACCCTTTTTCCTGATATACTGAGGACACTCGGTCTCTAGCAATTTCTT95170.2657204928658641No Hit
GTTTCTTGTTCTTTGAATGATGATGCTGACATTCATGCTCAAGCACATGC93070.2598571637178309No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG80670.2252356011294447No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT65780.18366180540839067No Hit
AAATAGATCTTAGGCAAAATACCAGCTGATGAAGGCATCTGATGCCTTCA50380.14066405832281426No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT48910.13655972791919102No Hit
ATTTAATGCCTCAAGTAAAAGACTGATCAATGGAAGCAAGCAAAACTAAA48750.13611299807934088No Hit
CCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAG46060.12860235264686035No Hit
ATCTTAAATAGATCTTAGGCAAAATACCAGCTGATGAAGGCATCTGATGC40610.1133856174769648No Hit
TTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTA40050.1118220630374893No Hit
GTTCAGTCATCTCCAAAAACAGTAAAAATAACCACTTTTTGTTGGGCAAT38430.10729892340900658No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT37140.10369716407521477No Hit
GTACAGGAGTAATAGCAATTCCCTGTAGCTCTCAAAGCAAATTTTGAGCT36780.10269202193555196No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT36190.10104470565110454No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)