FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004920946

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920946
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3581583
Sequences flagged as poor quality0
Sequence length150
%GC39

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT144300.40289447431484904No Hit
TCTTTATCACCAGAATGAGGAACTCCTGGAAGTTAACTGCACCATCAGTG125070.3492031316878598No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA77480.21632892494743244No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG73590.2054678057160758No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT70090.19569559046935392No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT44540.12435841916828395No Hit
CCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAG41100.11475372761150587No Hit
AAATAGATCTTAGGCAAAATACCAGCTGATGAAGGCATCTGATGCCTTCA41000.11447452146159953No Hit
TTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTA38180.10660090803424073No Hit
GTCTGTTTCTATCATTTGGTATTCTACTCCTTTAAAAATTTCATATTGCC37630.10506527420975585No Hit
ATCTTAAATAGATCTTAGGCAAAATACCAGCTGATGAAGGCATCTGATGC36090.1007654995011982No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)