FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004920975

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920975
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5208795
Sequences flagged as poor quality0
Sequence length125
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC150000.2879744739426297No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC147700.28355886534217606No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA134300.25783314566996784No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA118820.22811417995908845No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC116270.2232186139020637No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC103300.19831842105515768No Hit
TCCTGTAACAGTGCGAGACCACTTCATGCCTTGCAGGTGCTGCTGCTTCT99290.19061990345175803No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC85920.1649517786743383No Hit
ATATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82990.1593266772833256No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67130.12887817623845824No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC66380.12743830386874508No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA63710.12231235823256627No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC59600.11442185764653821No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG58210.11175329418800317No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC58140.11161890610016328No Hit
ATCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT57940.11123494013490644No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53270.10226933484615923No Hit
CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA52940.10163579100348547No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA52410.10061828119555484No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTATCA50300.086.0881351
ATACGGG46250.076.66971
AAGTACG38600.071.5471
ATATCAA160600.070.877761
ACCTAGT4150.051.2940861
CAACGCA374400.046.0352065
AACGCAG384700.045.093876
TATCAAC386950.044.473182
ACGCAGA389700.044.1703267
CGCAGAG390150.044.1337368
GTATCAA162500.043.0567132
GCAGAGT404150.042.4247449
TCAACGC407350.042.3664864
ATCAACG406400.042.3285063
ATATAAA29900.039.3441281
ATCTTAG28900.032.9520841
ATCTAGA20950.031.284821
AGAGTAC400550.030.99627310-11
ATACGAG6350.030.8763641
AAATAGG17350.030.6729771
Produced by FastQC (version 0.11.5)