FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004920976

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920976
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5208795
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA186500.35804826260200295No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA146350.28096709507669243No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC125380.24070826361951275No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC120670.2316658651377142No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT107040.20549858460546058No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC105990.2034827632878622No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC104380.2003918372675446No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC103860.19939352575787683No Hit
TCCTGTAACAGTGCGAGACCACTTCATGCCTTGCAGGTGCTGCTGCTTCT92690.17794902659828232No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC84220.16168806796965515No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG73770.14162584628498529No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC68740.13196910225877578No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC67200.12901256432629812No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA66050.1268047600260713No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC64790.1243857744449532No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT63390.12169801268815533No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA62630.12023894202017935No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC60980.11707122280681041No Hit
ATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCA58740.1127708039959338No Hit
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG58300.11192607887236874No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA53400.10251891272357617No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA222450.068.188271
CAACGCA325300.046.080415
ACGCAGA327150.045.6916287
GGTATCA124200.045.373331
CGCAGAG329400.045.3072628
AACGCAG334800.045.1283536
TATCAAC347050.043.671772
GCAGAGT344650.043.2503059
ATCAACG360450.041.8171163
TCAACGC362150.041.522234
AGAGTAC332200.035.07231510-11
TATTCTC39400.034.8889585
ATTTAGG83000.032.191451
TTTAGGG85250.031.2016122
CTATTGA84500.030.5627739
CAGAGTA336050.030.25258310-11
AGGGCTA86900.029.8565035
TCTCTGC44750.029.3875338
GGGCTAT91800.028.133216
TACGGGG126100.027.98131214-15
Produced by FastQC (version 0.11.5)