FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004920993

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920993
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5107215
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT315980.6186933583175958No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182710.35774879263943266No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159430.31216621974990283No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC60040.11755917853468083No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA276550.070.66031
GGTATCA119450.070.601441
TATCAAC419800.047.026272
ATCAACG437000.045.515323
TCAACGC441100.045.091824
CAACGCA454750.043.7770925
AACGCAG462450.043.1227576
ACGCAGA494750.040.2710347
CGCAGAG506000.039.4226578
GCAGAGT528450.037.8153429
AGAGTAC513850.032.97479210-11
CAGAGTA519450.027.43727910-11
AGTACTT230700.027.03210812-13
AGTACGG401400.026.60209312-13
GAGTACT220850.024.34984812-13
TACGGGT87450.023.56636414-15
TACTTTT273250.022.98544514-15
TACGGGC103050.022.65378814-15
TACGGGA148250.022.32644714-15
GTACTTT242000.022.26701514-15
Produced by FastQC (version 0.11.5)