FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004920995

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004920995
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2610029
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTACGCT129950.49788718822664424No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC69710.2670851549925307No Hit
TCATAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTACGCT65560.25118494851972906No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA62080.23785176333289784No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC60200.230648778231966No Hit
TGAAATACAGAGAAAAAGGCCTGTCTCTTATACACATCTCCGAGCCCACG52320.20045754280891134No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT50840.19478710772945435No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG47400.18160717754477057No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC45360.1737911724352488No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA45290.1735229761814907No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT44600.17087932739444658No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC44010.16861881611277116No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC38890.149002175837893No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA37740.14459609452615277No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT37000.1417608769864243No Hit
TGAAATACAGAGAAAAAGGCTGTCTCTTATACACATCTCCGAGCCCACGA36860.1412244844789081No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35720.13685671691770473No Hit
GGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGTTTCCCTTCGG34820.13340847936938632No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA32620.12497945425127462No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC32590.12486451299966397No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT32130.1231020804749679No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT31580.12099482419543998No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA30900.11838948915893271No Hit
TCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT30690.11758490039765841No Hit
TGAAATACAGAGAAAAAGGCATCCCAAAACAACCCCACGTACTGCAGTCA27450.10517124522371207No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC27340.10474979396780648No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG27230.1043283427119009No Hit
TGAAATACAGAGAAAAAGGCATCCCAAAACCTGTCTCTTATACACATCTC26970.10333218519794224No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC26640.10206783143022549No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)