FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004921021

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004921021
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5271992
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT308620.5853954254862298No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151690.2877280542155603No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144900.27484867200101976No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA130710.24793284967048507No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC113000.21434023420369377No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC111770.21200715023846775No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT91740.17401392111368907No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT88850.16853212220352384No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA82430.156354561994783No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC74840.1419577267947296No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73790.13996606975124393No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC68970.13082341551352883No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC63190.11985981769319831No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA57960.10993946880040789No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG57320.10872550641199759No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA339150.076.843041
GGTATCA192150.064.169881
CAACGCA490900.052.3687865
TATCAAC504200.051.7076642
ATCAACG510750.050.519613
AACGCAG516400.050.0197876
ACGCAGA523000.049.1820877
CGCAGAG524300.049.073878
TCAACGC529200.048.7038844
ACAGAGA31100.047.4535987
GCAGAGT547550.046.898079
AATACAG40150.039.6284524
ATTTAGG53950.037.3252331
TTTAGGG57600.035.3747332
TACAGAG43050.034.78296
CTATTGA56400.034.5912179
CAGAGAA42100.034.3707968
AGAGAAA42350.034.16799
TACTGTG20200.032.787797
GCTATTG63150.030.893828
Produced by FastQC (version 0.11.5)