FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004921022

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004921022
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5271992
Sequences flagged as poor quality0
Sequence length150
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147400.27959071258074747No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA118610.22498137326460282No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC103830.19694642935725246No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC96240.182549594157199No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT88000.1669198284064164No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT80720.15311100623824922No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA75990.14413906546140434No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75310.1428492304237184No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC70920.1345222071657165No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68620.13015952983236695No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC61040.11578166279463246No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC58020.11005327777432136No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG57240.1085737611134463No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA54120.10265569446994609No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA305700.076.596451
GGTATCA171800.064.333431
CAACGCA452900.051.5350275
TATCAAC457750.051.1305472
AACGCAG467600.049.8682566
ATCAACG468800.049.5879753
ACGCAGA475850.048.930347
CGCAGAG475950.048.648248
TCAACGC487500.047.818294
GCAGAGT483650.047.5462389
ATTTAGG51500.041.282281
TTTAGGG55700.037.8707772
CTATTGA58250.037.0798349
GCTATTG66250.032.8196148
GGCTATT67400.032.5797847
GGGCTAT71550.031.292376
TCTCTGC33650.030.8098688
TTCTCTG36200.030.0314757
AGGGCTA75550.029.540585
TATTCTC35800.029.360385
Produced by FastQC (version 0.11.5)