FastQCFastQC Report
Tue 6 Sep 2022
EGAF00004921033

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004921033
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78356502
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3880890.495286275030501No Hit
GTTTAGTAGTTGGACTTAGGGAACAAAGGAACCTTTAATAGAAATTGGAC2674820.3413654172566305No Hit
GTGTGGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAGCCAGGCCATCACTA2613300.33351412241449985No Hit
TCATAATATCCCCCAGTTTAGTAGTTGGACTTAGGGAACAAAGGAACCTT2315080.2954547409479816No Hit
GCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGTTGGACTTAGGGAA2012080.2567853271448998No Hit
GTATCAACGCAGAGTACGGGACATTTGCTTCTGACACAACTGTGTTCACT1806380.2305335171802335No Hit
ACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGAT1804700.23031911250964215No Hit
GACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGGATCCACGTGCAGCT1787090.22807169212326503No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1739970.22205815160048875No Hit
GTCCACTCCTGATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCA1698960.21682438044516075No Hit
TTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAG1678420.21420302810352612No Hit
GAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGTTGG1648940.21044073662195895No Hit
GTTATGGGCAACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGC1538340.19632576247469546No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1465410.18701830257813196No Hit
CTTCATAATATCCCCCAGTTTAGTAGTTGGACTTAGGGAACAAAGGAACC1446420.18459476406948336No Hit
TTACTGCCCTGTGGGGCAAGGTGAACGTGGATGAAGTTGGTGGTGAGGCC1377320.175776095773137No Hit
CTTGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAG1366480.17439267516051188No Hit
CCTAAGGAGAAGTCTGCCGTTACTGCCCTGTGGGGCAAGGTGAACGTGGA1364480.17413743150504601No Hit
GTACGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAAC1333680.17020667921087135No Hit
GATTACTGGTGGTCTACCCTTGGACCCAGAGGTTCTTTGAGTCCTTTGGG1276170.16286714789794982No Hit
GGCTGCTGGTGGTCTACCCTTGGACCCAGAGGTTCTTTGAGTCCTTTGGG1233100.15737047577749197No Hit
CTCCTAAGGAGAAGTCTGCCGTTACTGCCCTGTGGGGCAAGGTGAACGTG1135450.14490820429937007No Hit
CCCTTGGACCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCACTCCTG1022020.13043206037962235No Hit
GCACTGGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACC980490.12513192587387323No Hit
ATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGTTGGAC970980.12391824229213294No Hit
TATCAACGCAGAGTACGGGACATTTGCTTCTGACACAACTGTGTTCACTA945280.12063836131939631No Hit
CTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGTTGGACTTAGGGAAC932470.11900352570613731No Hit
TAGTGATACTTGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTG931510.11888100875151368No Hit
GACACAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTG902020.11511744105166921No Hit
TTATTAGGCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTT883590.11276537076655105No Hit
GTGCAGCTCACTCAGTGTGGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAG873240.11144448484951511No Hit
GAGTACGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAA855180.10913963464065815No Hit
CTCCTGATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCAAGAAA842490.10752011364672712No Hit
GCAAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACACC830620.10600524255153707No Hit
CTGATAGGCAGCCTGCACTGGTGGGGTGAATTCTTTGCCAAAGTGATGGG806340.10290658457418123No Hit
CCTTAGGGTTGCCCATAACAGCATCAGGAGTGGACAGATCCCCAAAGGAC804710.10269856099497651No Hit
CCTTTAATAGAAATTGGACAGCAAGAAAGCGAGCTTAGTGATACTTGTGG795750.10155506941848937No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)