FastQCFastQC Report
Sat 2 Jul 2022
EGAF00004921034

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004921034
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78356502
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAGTAGTTGGACTTAGGGAACAAAGGAACCTTTAATAGAAATTGGAC2634060.33616355155823574No Hit
GTGTGGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAGCCAGGCCATCACTA2416800.30843643326497655No Hit
TCATAATATCCCCCAGTTTAGTAGTTGGACTTAGGGAACAAAGGAACCTT2214950.282675967337082No Hit
GCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGTTGGACTTAGGGAA1900650.24256442688061802No Hit
ACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGAT1714650.21882676692229064No Hit
GTATCAACGCAGAGTACGGGACATTTGCTTCTGACACAACTGTGTTCACT1713760.2187131834956083No Hit
TTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAG1630050.20802996029608364No Hit
GTCCACTCCTGATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCA1629790.20799677862087307No Hit
GACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGGATCCACGTGCAGCT1614440.20603778356517244No Hit
GAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGTTGG1604780.20480495670927218No Hit
GTTATGGGCAACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGC1471170.18775340430587367No Hit
CTTCATAATATCCCCCAGTTTAGTAGTTGGACTTAGGGAACAAAGGAACC1359180.1734610358180614No Hit
TTACTGCCCTGTGGGGCAAGGTGAACGTGGATGAAGTTGGTGGTGAGGCC1320720.16855270032345243No Hit
GTACGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAAC1248420.15932564217836065No Hit
CTTGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAG1236740.15783501923043985No Hit
CCTAAGGAGAAGTCTGCCGTTACTGCCCTGTGGGGCAAGGTGAACGTGGA1228800.1568217019182403No Hit
GGCTGCTGGTGGTCTACCCTTGGACCCAGAGGTTCTTTGAGTCCTTTGGG1145290.14616400308426225No Hit
GATTACTGGTGGTCTACCCTTGGACCCAGAGGTTCTTTGAGTCCTTTGGG1112900.1420303320839922No Hit
CTCCTAAGGAGAAGTCTGCCGTTACTGCCCTGTGGGGCAAGGTGAACGTG1061140.1354246262805351No Hit
CCCTTGGACCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCACTCCTG967820.12351495731649685No Hit
ATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGTTGGAC930860.11879805456348727No Hit
GAACAAAGGAACCTTTAATAGAAATTGGACAGCAAGAAAGCGAGCTTAGT907990.11587934336323488No Hit
GCACTGGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACC906910.11574151178928331No Hit
TAGTGATACTTGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTG881300.11247311678104263No Hit
TATCAACGCAGAGTACGGGACATTTGCTTCTGACACAACTGTGTTCACTA876060.11180437840372201No Hit
TTATTAGGCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTT865710.11048349248668604No Hit
GACACAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTG863860.11024739210538011No Hit
CTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGTTGGACTTAGGGAAC856670.10932979116398023No Hit
CTCCTGATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCAAGAAA819650.10460523110130669No Hit
GCAAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACACC802010.10235398206009758No Hit
CCTTAGGGTTGCCCATAACAGCATCAGGAGTGGACAGATCCCCAAAGGAC793790.10130493063613279No Hit
GAGTACGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAA792440.10113264116869332No Hit
GTGCAGCTCACTCAGTGTGGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAG789680.10078040492415039No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)