FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004921035

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004921035
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3884815
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTACGCT251670.6478300768505063No Hit
TCATAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTACGCT105660.2719820634959451No Hit
TGAAATACAGAGAAAAAGGCCTGTCTCTTATACACATCTCCGAGCCCACG86950.22382018191342443No Hit
TGAAATACAGAGAAAAAGGCATCCCAAAACCTGTCTCTTATACACATCTC69800.17967393556707334No Hit
TGAAATACAGAGAAAAAGGCTGTCTCTTATACACATCTCCGAGCCCACGA56180.1446143510051315No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC55920.14394507846576993No Hit
TGAAATACAGAGAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGAC44860.1154752542913884No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA43850.11287538788848374No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC41030.10561635496156188No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG40530.10432929238586651No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)