FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004921038

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004921038
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7167578
Sequences flagged as poor quality0
Sequence length150
%GC42

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA708900.9890370219898548No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC663600.9258357565135671No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA542900.7574385657191314No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG541520.755513229154953No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC484580.6760721683112483No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA483860.6750676448864595No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA470670.6566653338129003No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC451790.6303244973406638No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC441200.6155496319677303No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT439340.6129546131203595No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC415700.5799727606731311No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC374610.5226451668890105No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA314350.4385721369198912No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC296210.41326372730090977No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA287420.4010001704899479No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC271900.3793471100000586No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA271650.3789983171442292No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC262920.36681847061866646No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG251450.35081585439321344No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA230950.3222148402152024No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC227590.31752706423285526No Hit
ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT216910.3026266334318231No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC211670.29531593517363885No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT197430.2754486941055961No Hit
CTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGC189920.2649709567164808No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT182750.2549675776112935No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC175510.24486653650647403No Hit
ATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCA173370.24188086966057432No Hit
GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG171900.23982996766829745No Hit
CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA169100.2359234876830081No Hit
GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG164400.22936618199341532No Hit
ATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGA163320.22785939685623235No Hit
AAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAA161200.224901633438799No Hit
GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA161180.22487373001033265No Hit
GTAATAGCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCA159790.22293444173192117No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA159470.22248798687645954No Hit
GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA157870.22025571259915136No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT155910.21752117660944884No Hit
ATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCA154440.21547027461717194No Hit
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG151270.21104758120525513No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG148130.2066667429360378No Hit
CCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCT145330.2027602629507485No Hit
GCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGT134730.1879714458635818No Hit
ATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGT133970.1869111155818604No Hit
ACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTC132410.18473464816148494No Hit
CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA130510.1820838224571815No Hit
TGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATG130220.1816792227444194No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC129740.18100954046122694No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA129420.18056308560576528No Hit
ATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGT126920.17707515704747126No Hit
GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG124290.17340585620414595No Hit
AACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTA123380.17213625020892692No Hit
GTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAA123290.17201068478082834No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT121550.1695830865042557No Hit
TAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGG119610.16687645394301953No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT119180.16627653023099295No Hit
ATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAAC113390.15819848768998399No Hit
AATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTA111610.15571508255647862No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC110610.15431991113316101No Hit
AAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGA109220.15238062285474954No Hit
ATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACT107300.14970189372197973No Hit
ATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAA106560.14866946686872468No Hit
TCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAG101300.14133086518207408No Hit
GTATTTGCATTATTGATGGTTTTTACTATGTTGGGTATGCTGTTCGATTT101150.14112158946857642No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC98440.13734067491138569No Hit
GTATGGGATTTCTAAAAATAATGTTAAGAATTTTTGCTGGTTTTTTGCGA98070.13682446148475816No Hit
GTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGG97470.1359873586307676No Hit
GCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAG96980.13530372463334198No Hit
GGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCT95590.1333644363549305No Hit
TCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTA94220.13145305150498537No Hit
CTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAG90530.12630486895294338No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87560.12216120982569006No Hit
GTCTGATATTGGGGAAATTTGGGAAGCAGTTAAAGTTTTAGAAAATAATG87450.12200774096912514No Hit
ATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTT87260.1217426583986948No Hit
ATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAA86560.12076603840237246No Hit
ATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATG85930.11988708040568236No Hit
GTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCA85350.11907788098015815No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT84420.11778037155647278No Hit
GTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAA82840.11557600070763094No Hit
TCTTTAATCCTCTCACTCCACAAATTCATAAGCTTCACCATTTTACTTCA79770.11129282443804588No Hit
CAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAGGAAGAAAT79420.1108045144398847No Hit
GGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAG78960.1101627355851586No Hit
CAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTAT77590.1082513507352135No Hit
CAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATAC76890.10727473073889116No Hit
TTCATAAGCTTCACCATTTTACTTCACCAATTCCTTAGAGATGTAATAGC76090.10615859360023708No Hit
CTCTCACAGCCAATAACTTCAACAACTTCCCTATCTTTAATCCTCTCACT75780.10572609045900862No Hit
ATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACT75030.1046797118915204No Hit
GTATTTCATCTTACCCAACCCCTTATGAAGATGTCAATTTAAACGCTATT74930.10454019474918864No Hit
ACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTAC72720.10145686590365673No Hit
ATGTAATAGCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAG72620.10131734876132495No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA109600.074.647131
GTATCAA202600.072.422081
TTCTCTG96700.063.0378077
TCTCTGC99950.059.404368
TATTCTC113100.053.5785065
AACGCAG293200.050.1225246
ATCAACG292300.050.0063633
ACGCAGA294500.049.6083687
CGCAGAG294350.049.5606468
CTCTGCA121000.049.4859739
GCAGAGT296250.049.0723889
CAACGCA300350.048.809525
ATTCTCT128450.048.4084056
TATCAAC311700.047.1954772
TCAACGC309200.047.156454
ACTGTGC74900.043.047058
CACTGTG67100.040.3282627
CTGTGCA68200.039.8888559
GCTATTG225200.039.116528
AGGGCTA233550.038.2721525
Produced by FastQC (version 0.11.5)