FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004921039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004921039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6791119
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA273230.40233428393759557No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA198730.29263218624206117No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC197970.29151307759442885No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197140.2902908931503041No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC176400.25975100716097005No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC173840.2559813780321034No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC146050.21506028682460138No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC138680.204207877965325No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT134070.19741960051060806No Hit
TCCTGTAACAGTGCGAGACCACTTCATGCCTTGCAGGTGCTGCTGCTTCT132750.19547588549103617No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG112620.1658342314425649No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA112480.16562807984958No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC109760.16162284890015916No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA97000.14283360371096426No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC96200.14165559460819344No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95860.14115494073951582No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95180.1401536330021606No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC89820.132260972013596No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC88980.13102406245568662No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC88280.12999330449076213No Hit
ATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCA84430.12432413568367748No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT78620.11576884457480424No Hit
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG74260.10934869496470316No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA72620.10693377630402294No Hit
TTTTAATACAGTAAGAATAGGTATCCTGAATAAATGAGAACTCTAGAAAA68580.10098483033503022No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA334950.062.4672051
GGTATCA195200.050.078591
CAACGCA499050.042.3635835
AACGCAG509750.041.774346
ACGCAGA514750.041.114337
TATCAAC519200.040.892042
CGCAGAG518450.040.808858
ATCAACG539900.039.236173
TCAACGC543450.038.946494
GCAGAGT545850.038.7603729
TATTCTC54100.035.256455
AGAGTAC527550.032.6211410-11
ATTTAGG112400.030.9811361
AGGGCTA114800.030.3309885
TCTCTGC60500.030.1489548
CTATTGA114550.030.083079
TTTAGGG116750.029.672362
GGGCTAT120700.029.092376
GGCTATT120700.028.846037
CAGAGTA534700.028.83476610-11
Produced by FastQC (version 0.11.5)