FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004921042

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004921042
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5901255
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA66640.11292513202700104No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN61400.10404566486281308No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA244400.071.866731
GGTATCA98200.066.8913961
TATCAAC352200.049.514012
CAACGCA357850.048.3071445
TCAACGC360900.047.940014
ATCAACG362250.047.7941973
AACGCAG363200.047.681866
ACGCAGA363650.047.580687
CGCAGAG376050.045.978178
GCAGAGT397900.043.48739
AGAGTAC379700.034.954910-11
CAGAGTA391550.030.34329210-11
AGTACGG436550.030.06349212-13
GAGTACG438800.026.82624612-13
TACGGGA197750.025.77737614-15
GTACGGG458650.025.08126414-15
TACGGGT76050.024.83240914-15
TACGGGG123100.024.4976414-15
TACGGGC121250.024.16010514-15
ACGGGAT47350.023.81071516-17
Produced by FastQC (version 0.11.5)