FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004921057

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004921057
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2584831
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156840.6067708101612832No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92420.3575475533990423No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72710.28129498601649394No Hit
GTGCAAGACGATGACTTGCAAAATGTCGCAGCTGGAACGCAACATAGAGA43190.16709022756226616No Hit
GTGTGGCTCCTCGGCTTTGACAGAGTGCAAGACGATGACTTGCAAAATGT34860.13486374931281775No Hit
CCCTGAACCAGGGGGAATTCAAAGAGCTGGTGCGAAAAGATCTGCAAAAT33250.12863510225620164No Hit
CTCTAAGGGTGAGCTGAAGCAGCTGCTTACAAAGGAGCTTGCAAACACCA30380.11753186185092952No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT29200.11296676649266432Clontech Universal Primer Mix Long (96% over 26bp)
GTCCAGGGGCCCAGCCTCTGGGCCCAGTAACTCAGCTACTCTTTGTGGCT27330.10573225096727794No Hit
CTTTATTATTCTGCAGGTACATGTCCAGGGGCCCAGCCTCTGGGCCCAGT27320.10569356371847907No Hit
GTCTTAGGGGGTGCCCTCCCCGAGGCCTGGCTTATGGTGGTGGCCAGGGC27070.10472638249850764No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)