FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004921058

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004921058
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2584831
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCAAGACGATGACTTGCAAAATGTCGCAGCTGGAACGCAACATAGAGA44480.1720808826573188No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36970.1430267588093767No Hit
GTCCAGGGGCCCAGCCTCTGGGCCCAGTAACTCAGCTACTCTTTGTGGCT32470.12561749684989076No Hit
GTGTGGCTCCTCGGCTTTGACAGAGTGCAAGACGATGACTTGCAAAATGT31270.12097502699402786No Hit
CCCTGAACCAGGGGGAATTCAAAGAGCTGGTGCGAAAAGATCTGCAAAAT27250.10542275297688708No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA25880.10012259989144359Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)