FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004921077

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004921077
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5165396
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196510.38043549807217103No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA164310.3180975863225201No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC153790.29773128720431113No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC150530.29142005762965706No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC120220.23274111026531172No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA118370.22915958427969513No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106430.2060442219725264No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC96800.18740092724739787No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT96380.1865878240506633No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT92620.1793086144798966No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC83660.16196241294955893No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83460.16157522095111393No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC82610.15992965495772252No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT70840.13714340584923207No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA68530.1326713382671919No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA64000.12390143950241181No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT58480.1132149403453288No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC56820.11000124675823499No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG55630.10769745436748701No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54420.10535494277689454No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT53350.1032834655852136No Hit
TCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT53230.10305115038614658No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA168150.082.8429951
GGTATCA87500.069.45721
ATCAACG253150.054.6525043
TATCAAC259250.053.865822
CAACGCA257450.053.560255
ACGCAGA259250.053.2845087
CGCAGAG262300.052.6100358
AACGCAG267300.052.245336
GCAGAGT268300.051.4603429
TCAACGC279200.049.501844
TTCTCTG30350.041.0328947
CTATTGA73350.038.1763349
TTTAGGG75700.037.5461962
CACTGTG71300.036.245137
ACTGTGC89550.036.1735958
GCTATTG77800.035.90028
AGGGCTA81700.035.1655585
CTGTGCA79850.034.0770239
GGGCTAT88200.032.5648466
GGCTATT88250.032.220127
Produced by FastQC (version 0.11.5)