FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004921089

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004921089
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9746728
Sequences flagged as poor quality0
Sequence length150
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT236730.24288150854317472No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224880.2307235823139827No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA127520.130833650020807No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123360.12656555102389233No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG100450.10306022698078782No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA181850.066.803671
CAACGCA205100.057.218175
AACGCAG209250.056.1521876
TCAACGC212350.055.5358774
ATCAACG217450.054.266193
CGCAGAG227700.051.6020438
ACGCAGA229550.051.1237037
GGTATCA85900.048.4590871
TATCAAC270550.044.069282
GTCTCGC11750.043.0532651
GCAGAGT276800.042.6307229
GGGTTAG93950.037.7800187
GAGTACG190150.037.777741
AGTACGG195500.036.4964032
TAGGGGT102250.035.6286354
TACGGGT47950.035.435244
CGCTCTG11600.035.3776445
CTCGCTC14150.034.5988963
ACGGGTA18450.034.3398445
GGGGTTA104900.034.316786
Produced by FastQC (version 0.11.5)