FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004921090

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004921090
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9746728
Sequences flagged as poor quality0
Sequence length150
%GC39

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT195710.20079559006879028No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177580.18219447593079444No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA116700.11973248868748568No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109120.111955519842146No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA159500.065.4900051
CAACGCA186850.054.7539565
AACGCAG185550.054.2844926
TCAACGC187600.054.073144
ATCAACG194250.052.2582443
CGCAGAG199300.050.250328
ACGCAGA203050.049.464117
GGTATCA81200.046.43891
GCAGAGT238350.041.9873669
TATCAAC249750.041.596152
GTCTCGC10550.038.344491
GAGTACG188600.037.689541
TAGGGGT92100.036.8965534
AGTACGG194100.036.7570272
GGGTTAG90100.036.1187447
ATAGGGG97250.035.0901683
GGGGTTA97900.034.050016
CGCTCTG12650.033.0104945
TACGGGG98300.032.7375562
GTACGGG241050.032.365631
Produced by FastQC (version 0.11.5)