FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004921094

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004921094
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences946219
Sequences flagged as poor quality0
Sequence length150
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52520.5550512090752775No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31910.33723693986275904No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28910.3055318060618102No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23020.24328406003261402No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC20730.2190824745645564No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAAAACCACGGCACTCAGG16170.1708906711871142No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15620.1650780633236069No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14820.15662336097668722No Hit
GTCCTGGGCCCAGTCTATACTGACGCAGCCGCCCTCAGTGTCTGGGGTCC13100.13844575093080988No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT13060.1380230158134639No Hit
GTCTATACTGACGCAGCCGCCCTCAGTGTCTGGGGTCCCAGGGCAGAGGG12910.13643775912341646No Hit
AGTCTATACTGACGCAGCCGCCCTCAGTGTCTGGGGTCCCAGGGCAGAGG10980.11604078971147272No Hit
CTCCTGTACTGGGAGCACCTCCAACATCGGGGCAGGTTATGATGTACACT10090.10663493335052457No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAAAACCAC9800.10357010374976616No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA9670.10219621461839173No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC9620.10166779572170924No Hit
GATGTACACTGGTACCAGCAACTTCCAGGAACAGCCCCCAAACTCCTCAT9530.10071664170768078No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)