FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004921141

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004921141
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5263406
Sequences flagged as poor quality0
Sequence length125
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA86590.16451324484563798No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC58920.11194272302003684No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC54510.10356411798747808No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52700.050.7345851
GTATCAA96350.048.9633371
GGCGTCG2450.048.4976548
CAACGCA107850.043.8484155
AACGCAG113550.041.856196
TCAACGC117100.040.5888184
ATCAACG116750.040.559763
CGGGCGT3150.037.7203986
TATCAAC126900.037.6440732
CGCAGAG128150.036.8557558
ACGCAGA128500.036.755377
ACGGGCG3700.032.1136175
GCAGAGT147850.031.944989
GTACATG177950.028.480871
ACATGGG162100.028.332863
GGGCGTC5100.027.9574727
TACATGG177250.027.621242
AGAGTAC150300.026.91915910-11
CGCGATC900.00960288826.4042788
CTCGTCG1150.00101424825.830279
Produced by FastQC (version 0.11.5)