FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004921142

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004921142
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5263406
Sequences flagged as poor quality0
Sequence length125
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA83660.1589465072616477No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC57120.10852288423123735No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA89850.047.225591
GGTATCA48600.046.2872621
CAACGCA90650.044.768545
AACGCAG95950.042.3512346
ATCAACG99900.040.9250223
TCAACGC100050.040.9227564
CGCAGAG107850.037.398888
TATCAAC111300.037.322112
ACGCAGA108750.037.2563177
GGCGTCG2350.032.9097448
GCAGAGT125150.032.2753839
GTACATG184400.028.7878911
ACATGGG167900.028.4972553
TACATGG181500.028.034672
CGGGCGT3000.027.7649616
AGAGTAC126650.025.88062710-11
ACGGGCG3550.025.1431275
CATGGGG59650.023.943874
CATGGGA85250.023.5249714
CAGAGTA134400.023.23744810-11
Produced by FastQC (version 0.11.5)