FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004921154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004921154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10441749
Sequences flagged as poor quality0
Sequence length150
%GC40

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGCTATAATTTTTCATCT690500.6612876827435711No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT428050.40994090166312175No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA399370.3824742387506154No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCATAATATAG313080.29983482652187865No Hit
ATATTATGCTTGGCTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT304650.29176146639801437No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCATAATAT291450.27911990606171433No Hit
CCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAG214050.2049943931806827No Hit
TTGCTATATTATGCTTGGCTATAATTTTTCATCTTTCCCTTGCGGTACTA165690.1586803130395109No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA150350.14398928761838653No Hit
CTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACGTTCTCCTGA147920.14166209128374949No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT123030.11782508849810505No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT121210.11608208548203945No Hit
TCTGAGATGTTAGTATTAGTTAGTTTTGTTGTGAGTGTTAGGAAAAGGGC117260.11229919432079818No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGAGTGATAT114080.10925372751250773No Hit
GTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATT113180.10839180294412362No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGC110350.066.0245742
GTACATG114600.065.998871
CATGCTA111050.064.834774
CGGGACA12050.062.7377324
ACATGCT126200.057.1653483
CCGCAGT24200.055.5228841
TAAGACT133050.053.7895669
ACGGGAC19150.053.764023
CAACGCA114450.050.8309635
AACGCAG114000.050.778986
CGCAGAG113000.050.6541758
TGCTAAG144750.049.6912276
TCAACGC117900.048.9153634
ACGCAGA118300.048.7499667
ATCAACG119650.048.139523
TTGTACG21550.048.110719
GGGGTTA239050.047.0763746
CGGGGAC6550.045.0680854
AGGGGTT245300.043.4994165
TAGGGGT248650.043.376044
Produced by FastQC (version 0.11.5)