FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004921177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004921177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6953225
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189360.27233406081350736No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA138770.19957645552962835No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA128530.18484947632213827No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC121590.17486849627331202No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC107610.15476271801933636No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC96500.13878452085183496No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC91780.1319963038733825No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88010.12657435937999995No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC83980.12077848768017718No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81610.11736999737531864No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG80290.11547159771185313No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC74520.10717329009200767No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC71890.10339087258070896No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA220800.067.493241
GGTATCA91350.058.6068041
TCAACGC306050.048.9606324
ATCAACG306200.048.9373
TATCAAC307400.048.861932
AACGCAG308650.048.6612476
CAACGCA308750.048.551375
ACGCAGA327850.045.7749067
CGCAGAG332550.045.127968
GCAGAGT366800.040.865549
AGAGTAC351150.033.74509410-11
AGTACGG275150.029.40877212-13
CAGAGTA371900.027.31739210-11
TTAGGGC77850.026.1014673
GAGTACG276350.024.69112412-13
TACGGGC67700.024.55415214-15
TACGGGA109450.024.45279314-15
TAGGGCT83900.024.4315764
TACGGGT59250.023.53890414-15
GGGCTAT77400.023.1813346
Produced by FastQC (version 0.11.5)