FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004921178

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004921178
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6953225
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA143680.20663792700509476No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA118380.17025193345533907No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC118220.17002182440522204No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC114550.1647436980681626No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC105010.15102344595493458No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC85300.1226768873436427No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC84170.12105174217719115No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG76040.10935932606811946No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC71880.10337649076507663No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC70370.10120483660459716No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN69920.10055765490114299No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA176850.065.364631
CAACGCA229900.049.8024755
AACGCAG230800.049.685926
TCAACGC230750.049.6798864
ATCAACG229900.049.6062363
ACGCAGA238150.048.1077427
TATCAAC242400.047.442552
CGCAGAG244750.046.736578
GGTATCA77150.046.346141
GCAGAGT273550.041.7761769
AGAGTAC255700.034.27621510-11
AGTACGG288100.030.19369112-13
CAGAGTA275700.027.14204410-11
TTAGGGC81750.026.7073883
TAGGGCT86300.025.9189644
GAGTACG289950.025.58188812-13
TACGGGC78100.024.67201414-15
GGGCTAT80400.024.4002786
AGGGCTA82500.023.719265
TACGGGT64700.023.62324114-15
Produced by FastQC (version 0.11.5)