FastQCFastQC Report
Tue 6 Sep 2022
EGAF00004921188

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004921188
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64249931
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT2410840.37522841853324324No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT2372450.36925331484013574No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT2221250.34572021563727434No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA1674430.26061195303073553No Hit
GGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGC1547030.2407831379616579No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC1525850.23748663636697134No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA1499390.2333683440064706No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTT1379630.21472863527277564No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT1315550.20475508370584863No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT1294800.20152550825307503No Hit
CATTAGTTCTTCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGT1276470.19867258690129957No Hit
CTTCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTAT1244620.19371538313403014No Hit
CTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT1211310.18853094176863786No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG1171330.18230836699264313No Hit
ACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTG1168590.18188190739068655No Hit
ATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGC1155880.17990369514949361No Hit
GGCTTATGCGGAGGAGAATGTTTTCATGTTACTTATACTAACATTAGTTC1154560.17969824745804008No Hit
ACTTATACTAACATTAGTTCTTCTATAGGGTGATAGATTGGTCCAATTGG1117160.17387722953352275No Hit
AGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATC1088280.16938228307202385No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAG1087950.16933092114916046No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA979220.15240794577662659No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC916530.14265073685448781No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG903660.1406476218628157No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG899080.1399347806303481No Hit
CCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAG879060.13681882397663586No Hit
TACTTATACTAACATTAGTTCTTCTATAGGGTGATAGATTGGTCCAATTG863090.1343332181944289No Hit
ATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTA815940.1269946889125842No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTG793250.12346316761025006No Hit
TCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTA749050.11658378279036595No Hit
GTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAAC738660.1149666604311217No Hit
CGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACC721390.11227871980127108No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT720190.11209194917267692No Hit
ATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAA711010.11066315386393177No Hit
GTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTA707670.1101433089476781No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA685120.10663357755201326No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT679760.10579933541095943No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAAC659730.10268182233534226No Hit
GTGTTGGGTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGAT648130.10087637292559894No Hit
GTTTTCATGTTACTTATACTAACATTAGTTCTTCTATAGGGTGATAGATT647200.10073162568843849No Hit
GATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCC643350.1001324032550323No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)