FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004921190

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004921190
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4420209
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA183350.069.6870651
GGTATCA79350.060.6740041
ATCAACG252950.049.855393
CAACGCA254550.049.6366585
TCAACGC254600.049.6257864
TATCAAC262250.048.5639572
AACGCAG260850.048.437846
ACGCAGA265800.047.5123147
CGCAGAG279050.045.3202938
GCAGAGT293400.043.0804989
AGAGTAC279550.034.44198210-11
CAGAGTA285250.030.53065910-11
AGTACGG312850.029.84054612-13
GAGTACG313350.026.70729812-13
TACGGGT63250.024.88100414-15
GTACGGG331650.024.64060814-15
TACGGGC90250.024.42551614-15
TACGGGG104250.023.99897614-15
TACGGGA121050.023.81396714-15
GTATATA26100.019.8371731
Produced by FastQC (version 0.11.5)