FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004921217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004921217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1066025
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGA137691.2916207406017681No Hit
TCATAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGA75130.7047677118266457No Hit
TGAAATACAGAGAAAAAGGCATCCCAAAACCTGTCTCTTATACACATCTC47350.4441734480898666No Hit
TGAAATACAGAGAAAAAGGCCTGTCTCTTATACACATCTCCGAGCCCACG46100.4324476442860158No Hit
TGAAATACAGAGAAAAAGGCATCCCAAAACAACCCCCCGTACTCTGCGTT37840.35496353275017006No Hit
TGAAATACAGAGAAAAAGGCATCCCAAAACAACCCCACGTACTGCAGTCA35750.33535798879013157No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC34160.3204427663516334No Hit
TGAAATACAGAGAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGAC33200.31143734903027603No Hit
TGAAATACAGAGAAAAAGGCTGTCTCTTATACACATCTCCGAGCCCACGA29710.27869890480992476No Hit
TCATGTAACAAGCAAAATGAAACCACCAGAAACCAGGAACGTACTGCAGT29040.2724138739710607No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT25570.23986304261157101No Hit
TCATAAAAAGGTCAATCCAACCACCCAAATCAAGCGTTACGTACTGCAGT23220.21781853146033162No Hit
TGAAATACAGAGAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACG21170.19858821322201636No Hit
GGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGTTTCCCTTCGG21050.1974625360568467No Hit
TCAGGTAACAAGCTAAATGAAACCACCAGAAACCAGGAACGTACTGCAGT20480.19211556952229075No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA19660.18442344222696466No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT19660.18442344222696466No Hit
TGAAATACAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAG17890.167819704040712No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG17190.16125325391055556No Hit
TCACCCAAGAACCCAACTAAAGAAACGATAAAACCACGTACTGCAGTCAT17080.1602213831758167No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT16620.15590628737599962No Hit
TCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT16430.15412396519781432No Hit
TGAAATACAGAGAAAAAGGCATCCCAAAACAACCCCATATGTGTATAAGA15720.14746370863722708No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT15150.14211674210267114No Hit
ATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTC15040.14108487136793227No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC14420.13526887268122229No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC14380.13489364695949907No Hit
TCATAAAAAGGTCAATCCAACCACTGTCTCTTATACACATCTCCGAGCCC14280.133955582655191No Hit
TCATAAAAAGGTCAATCCCTGTCTCTTATACACATCTCCGAGCCCACGAG13960.13095377688140522No Hit
TCATAAAAAGGTCAATCCAACCACCCAAATCAAGCGTTACGTACTAGCAT13640.1279519711076194No Hit
CGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGC13510.12673248751201893No Hit
TCATAAAAAGGTCAATCCAACCACCTAAATCAAGCGTTACGTACTGCAGT13290.12466874604254122No Hit
TGAAATACAGAGAAAAAGGCATCCCAAAACAACCCCCGTACTCTGCGTTG13120.12307403672521752No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA12650.11866513449496964No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA12400.11631997373419947No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA12260.11500668370816819No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12260.11500668370816819No Hit
GACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTAATTGATGGGG11950.11209868436481321No Hit
TGAAATACAGAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGTA11770.11041016861705871No Hit
TCATGTAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGA11710.10984733003447386No Hit
GGTGGAGACAGCCTGGCCATCATTACGCCATTCGTGCAGGTCGGAACTTA11630.10909687859102743No Hit
TGAAATACAGAGAAAAAGGCATCCCAAAACAACCCCCCGTACCTGTCTCT10880.10206139630871695No Hit
ACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATGCAGTGTACCCGC10870.10196758987828615No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC10820.10149855772613213No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAG10700.10037288056096245No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)