FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004921218

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004921218
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1066025
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTTCACTGTCTCTTATACACATCTGACGCTGCCGACGATCTACTCTG131351.2321474637086371No Hit
TTTTATGACTGTCTCTTATACACATCTGACGCTGCCGACGATCTACTCTG61310.5751272249712718No Hit
GTTTTGGGATGCCTTTTTCTCTGTATTTCACTGTCTCTTATACACATCTG45370.42559977486456696No Hit
GCCTTTTTCTCTGTATTTCACTGTCTCTTATACACATCTGACGCTGCCGA42860.40205436082643464No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC37480.351586501254661No Hit
TTTTCTCTGTATTTCACTGTCTCTTATACACATCTGACGCTGCCGACGAT31080.29155038577894515No Hit
CCTTTTTCTCTGTATTTCACTGTCTCTTATACACATCTGACGCTGCCGAC27580.25871813512816305No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT26560.2491498792242208No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT20900.19605543960038463No Hit
GGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGTTTCCCTTCGG20410.19145892450927512No Hit
TTTCTCTGTATTTCACTGTCTCTTATACACATCTGACGCTGCCGACGATC19640.18423582936610305No Hit
CTGTATTTCACTGTCTCTTATACACATCTGACGCTGCCGACGATCTACTC16800.15759480312375415No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA16620.15590628737599962No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG15970.14980886939799723No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT15940.14952745010670482No Hit
GGTATCAACGCAGAGTACGGGGGGTTGTTTTGGGATGCCTTTTTCTCTGT14550.13648835627682276No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC14470.13573790483337633No Hit
ATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTC13400.1257006167772801No Hit
TGGTTGGATTGACCTTTTTATGACTGTCTCTTATACACATCTGACGCTGC13360.12532539105555687No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT13210.12391829459909476No Hit
TCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT13150.12335545601650993No Hit
GGATTGACCTTTTTATGACTGTCTCTTATACACATCTGACGCTGCCGACG12830.12035365024272414No Hit
GACGTATACGGTGTGACGCCTGCCCGGTGCCGGAAGGTTAATTGATGGGG12800.12007223095143171No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA12060.11313055509955207No Hit
CGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGC11890.11153584578222836No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA11890.11153584578222836No Hit
GGGCTACACACGTGCTACAATGGCGCATACAAAGAGAAGCGACCTCGCGA11150.10459416993034872No Hit
TTACATGACTGTCTCTTATACACATCTGACGCTGCCGACGATCTACTCTG10940.10262423489130179No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT10940.10262423489130179No Hit
GTACGGGGGGTTGTTTTGGGATGCCTTTTTCTCTGTATTTCACTGTCTCT10890.10215520273914777No Hit
TCTCTGTATTTCACTGTCTCTTATACACATCTGACGCTGCCGACGATCTA10880.10206139630871695No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC10800.10131094486527051No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10710.10046668699139326No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)