FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004921219

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004921219
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4822114
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152490.31623059927658287No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT112330.23294762421626697No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107350.2226202035041063No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT103620.21488500686628315No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG102090.211712124599294No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84320.1748610671585118No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT80060.16602676751316953No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT78540.16287462303877512No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA69720.14458388997024957No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA64740.13425646925808887No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA62450.12950751475390254No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC54410.1128343295077636No Hit
ATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCG52200.10825127734433487No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73600.084.22111
GTATCAA176900.059.612691
AAGTACG12250.050.5528031
ATCAACG264400.044.539363
CAACGCA269250.043.7370765
TATCAAC272000.043.513652
AACGCAG273200.043.170056
TCAACGC274150.042.9770474
GCATGCA9300.042.2580071
ACGCAGA289150.040.6228187
CGCAGAG293900.039.9955678
GCAGAGT303700.038.6266149
CTCTGCA21000.037.6753129
ATACGGG17800.036.4631771
ATGTCCG6700.035.0712654-55
TCTATAC53850.034.366423
AGAGTAC311650.031.36136210-11
TCTCTGC24500.031.3219038
TACCAAC50050.030.6733867
CAGAGTA310350.030.06472810-11
Produced by FastQC (version 0.11.5)