FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004922108

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922108
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4139564
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA662571.6005791914317546No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC412780.9971581548201695No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC365390.8826774993694988No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA356120.8602838366552613No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC334010.8068724145827919No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG331290.8003016742826056No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC308250.7446436387986755No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC278790.6734767236356293No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT273510.6607217571705619No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA262450.6340039675675989No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA260560.6294382693443078No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC228840.5528118420200775No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA218960.5289445941649894No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG218720.5283648229620318No Hit
ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT192470.46495234763854354No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA178010.43002113266034775No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC172960.4178217802647815No Hit
AAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAA172780.4173869518625633No Hit
GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG172130.41581673818788645No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA170950.4129661964400116No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC164120.3964668742891763No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA161500.3901377053235558No Hit
CTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGC160620.38801187757937794No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT156620.37834902419675115No Hit
ATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCA152780.36907268494942946No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC150120.36264688744998264No Hit
CCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCT149070.3601103884370431No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA146340.35351549100340035No Hit
ATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATG135730.32788477240598285No Hit
GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG134470.32484097359045544No Hit
GCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGT127010.3068197520318565No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG124980.30191585394017345No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT121600.2937507428318538No Hit
AATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTA118550.2863828171276009No Hit
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG112300.2712846087172466No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT110230.2662840820917372No Hit
ACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTC109800.2652453253531048No Hit
CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA109460.26442398281558155No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC106190.25652460017528417No Hit
AACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTA106140.2564038145080013No Hit
ATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGT102110.24666848972500482No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA100330.24236851996973593No Hit
AATTGGTAGAGTTCCAACATCTCCTGGAAAAACCTTTGCTGGATATTTGT100240.24215110576862683No Hit
ATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAA98300.23746462187805287No Hit
GTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGG98240.23731967907731347No Hit
ATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGA97440.23538710840078808No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT95820.23147365278082427No Hit
GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA95250.23009669617379994No Hit
GTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAA93270.22531358374939967No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91180.2202647428569772No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT89540.21630297297010023No Hit
TAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGG89380.21591645883479518No Hit
ATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGT88730.21434624516011833No Hit
TCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTA85740.2071232622566048No Hit
AAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGA84270.20357216363848948No Hit
GTTCCAACATCTCCTGGAAAAACCTTTGCTGGATATTTGTTAAATATCAA83190.20096319322518022No Hit
ATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAA82620.19958623661815592No Hit
TGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATG79290.19154191117711913No Hit
CAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATAC78800.19035821163774735No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC78720.19016495457009483No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT75040.1812751294580782No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73480.17750661663885375No Hit
ATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACT73210.17685437403552645No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT72850.17598471723109002No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC72630.17545326029504557No Hit
ATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACT70510.17033194800225337No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT69930.1689308342617725No Hit
CTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTA68350.16511400717563493No Hit
CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA64860.15668316759929304No Hit
CTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATGGTGATTGT62500.15098208410354327No Hit
TATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTT62300.15049894143441192No Hit
GGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCT61410.1483489565567775No Hit
AACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCC59900.14470122940483587No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC58830.1421164161249832No Hit
CTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAG58170.1405220453168498No Hit
CATTAATAGCATGATGGTGATTGTTTTGAAAACCATAGGAGGAAACCTCC58010.1401355311815447No Hit
CTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTT54480.1316080630713766No Hit
CCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTT54210.1309558204680493No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC54040.13054514919928767No Hit
GGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAG53840.13006200653015632No Hit
TTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCC53420.1290474069249805No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC52920.12783955025215216No Hit
TAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGT52320.12639012224475815No Hit
GTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAA52260.12624517944401875No Hit
TCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAG51820.1251822655719298No Hit
GTATAGTTGGGATTTTAATATCATTAATAGCATGATGGTGATTGTTTTGA51540.12450586583514592No Hit
GTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATAC50950.12308059496120846No Hit
GCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTGG50840.12281486649318625No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC49300.11909466794087493No Hit
ATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAA49260.11899803940704867No Hit
GGGATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATG49220.1189014108732224No Hit
CATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTA49150.11873231093902643No Hit
GATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATAT47570.11491548385288886No Hit
GTATTTCATCTTACCCAACCCCTTATGAAGATGTCAATTTAAACGCTATT47500.11474638391869289No Hit
GCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTGGAAAAACCTT47470.11467391251832318No Hit
CTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATAT47280.11421492698264842No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA46490.11230651343957963No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG45560.1100599000281189No Hit
GTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGGGATTTTAATATC45170.1091177718233128No Hit
CAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTAT44520.10754755814863595No Hit
ATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTC43620.10537341613754492No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43290.10457623073347821No Hit
ATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGG43150.10423803086508628No Hit
AACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAG42700.10315095985954077No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT42550.10278860285769226No Hit
ATCATTAATAGCATGATGGTGATTGTTTTGAAAACCATAGGAGGAAACCT41860.10112176064918915No Hit
CTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTC41770.10090434644808005No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC41440.10010716104401335No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)