FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5168277
Sequences flagged as poor quality0
Sequence length125
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA84610.16371026552949852No Hit
GTATTGGTTAGCTCAACGCCTCACAGCGCTTACACACCCAACCTATCAAC82710.16003399198611065No Hit
TCGTGAGACAGTTCGGTCCCTATCTGCCGTGGACGTTTGAGATTTGAGAG67900.1313784071558084No Hit
GTATCAACGCAGAGTACGGGCAATTAGTATTGGTTAGCTCAACGCCTCAC53070.10268412470925997No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA354700.069.199161
GGTATCA158650.058.4339751
CAACGCA539250.045.0561035
TATCAAC571700.042.8179362
AACGCAG571600.042.60746
ACGCAGA573050.042.375877
CGCAGAG579550.041.8698
TCAACGC592000.041.126974
ATCAACG593300.041.0974243
GCAGAGT599450.040.531439
AGAGTAC606800.031.14607610-11
ATTTAGG33500.028.9521521
CAGAGTA584950.028.57775910-11
TACGGGG169450.026.0998814-15
TACGGGT154000.025.7634614-15
AGTACGG732600.025.69134112-13
TTTAGGG37550.025.3536012
TACGGGC188350.025.26524414-15
CTATTGA39800.023.4622349
TATTCTC24050.023.0051945
Produced by FastQC (version 0.11.5)