FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922126

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922126
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4186213
Sequences flagged as poor quality0
Sequence length125
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151550.36202171270310424No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86220.2059618084411854No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA57490.13733176023293606No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT54060.12913819722025613No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT45600.10892900098489972No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45480.10864234571914998No Hit
TCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT42370.10121319674846932No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA355550.047.17571
TATCAAC412950.040.503062
GGTATCA218600.038.365331
ATCAACG446850.037.882893
GCGCAGA54400.037.7216071
CAACGCA452600.037.494055
AACGCAG465250.036.5513086
TCAACGC466000.036.5307774
ACGCAGA538500.031.557297
CGCAGAG556700.030.5255978
CATGGGT136650.029.3811824
GCAGAGT589150.028.8947479
TATACGA7150.028.284132
ATGGGTG48100.027.8235535
ATGGGAG46750.027.7363955
GTCCTAG2404.3837645E-1027.2616041
GAGTACT107150.027.11720712-13
GTACATG673150.025.8984581
GCTTAGG7350.025.8959921
TACATGG672750.025.8166032
Produced by FastQC (version 0.11.5)