FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922127

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922127
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4186213
Sequences flagged as poor quality0
Sequence length125
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA56920.13597014772062482No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT53440.12765714501388248No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA298300.043.2169881
TATCAAC357850.035.7588122
GGTATCA192850.033.1926161
CAACGCA392200.032.4301535
ATCAACG397550.032.232773
CATGGGT133600.032.1940424
AACGCAG400800.031.7301226
TCAACGC408350.031.3511454
ATGGGTG47000.030.3782165
ATGGGAG47750.029.5273085
GTACATG670500.027.8332961
TACATGG672100.027.7578532
ACGCAGA465050.027.2932577
CGCAGAG466550.027.2630568
ACATGGG663100.027.1477343
TATACGA8950.026.587692
GCAGAGT487900.026.0944279
CATGGGG176350.025.536664
ATGGGTC43800.024.1765735
ATATACG9900.024.0366341
Produced by FastQC (version 0.11.5)