FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004922158

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922158
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2982383
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT235870.790877630404948No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121640.4078617669159192No Hit
TTATTACACCTCCTGATTTTCACTCCTTGAGTTTTATTTTCTCCCCTTGT108610.36417187195608347No Hit
GTGTAAGAAATTGCCATTTGAAACAATCCATTTTGTAACTGAACCTTATG101500.3403318755505245No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT90630.3038845111442762No Hit
TTCATACTCTAAGTATCAAAATCTTCCAATTATCATGCTCACCTGAAAGA80060.2684430537593595No Hit
TAACAGACTTGGAAGCAGATGATACAGACTTCTTTTTTTCATAATCAGGT75160.2520132390776101No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74090.24842550403486072No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62470.20946337207528343No Hit
TATTTGGACATAACAGACTTGGAAGCAGATGATACAGACTTCTTTTTTTC59130.19826427390445828No Hit
CTCTTAGGAATACAGTTTCTAGCATTAAACAAATAAACAAGGGGAGAAAA56060.18797049205283156No Hit
GGACATAACAGACTTGGAAGCAGATGATACAGACTTCTTTTTTTCATAAT55670.1866628129251005No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG53960.1809291429035104No Hit
TTTTATTACACCTCCTGATTTTCACTCCTTGAGTTTTATTTTCTCCCCTT52890.177341407860761No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA48260.16181690949820998No Hit
AGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTTACAAA46780.15685443485964076No Hit
AGATGATACAGACTTCTTTTTTTCATAATCAGGTTAGTGTAAGAAATTGC45400.15222726256151542No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT45000.15088605319973994No Hit
AATCAGGTTAGTGTAAGAAATTGCCATTTGAAACAATCCATTTTGTAACT42990.14414647615681822No Hit
CTATTTAATTGCTCAGACTGTGCTAGAGAATACGTACCATGAAATACATA42890.14381117381637437No Hit
ATTTAATTGCTCAGACTGTGCTAGAGAATACGTACCATGAAATACATATA41360.13868104800758319No Hit
ATACAGACTTCTTTTTTTCATAATCAGGTTAGTGTAAGAAATTGCCATTT41090.13777573168838475No Hit
GGTTAGTGTAAGAAATTGCCATTTGAAACAATCCATTTTGTAACTGAACC39620.13284678728385993No Hit
TTCTTACACTAACCTGATTATGAAAAAAAGAAGTCTGTATCATCTGCTTC37060.12426304736849693No Hit
CATATATTTCATAAGGTTCAGTTACAAAATGGATTGTTTCAAATGGCAAT36940.1238606845599643No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36870.12362597292165359No Hit
GACTGTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTC36070.12094355419810265No Hit
GATGATACAGACTTCTTTTTTTCATAATCAGGTTAGTGTAAGAAATTGCC35900.12037354021934808No Hit
ATATTTGGACATAACAGACTTGGAAGCAGATGATACAGACTTCTTTTTTT34350.11517635394246814No Hit
ATTATATGTTCATACTCTAAGTATCAAAATCTTCCAATTATCATGCTCAC33770.11323160036789373No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT33600.11266158638913915No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA32720.10971092579323313No Hit
GTTCATACTCTAAGTATCAAAATCTTCCAATTATCATGCTCACCTGAAAG32480.10890620017616785No Hit
GAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTTACAAAATG32460.10883913970807907No Hit
TACTCTAAGTATCAAAATCTTCCAATTATCATGCTCACCTGAAAGAGGTA31530.10572082794195112No Hit
ATTAAAATATATTTGGACATAACAGACTTGGAAGCAGATGATACAGACTT30110.10095953470764822No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30090.10089247423955945No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)