FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004922211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5607547
Sequences flagged as poor quality0
Sequence length150
%GC40

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGCTATAATTTTTCATCT404610.7215454458072309No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT249830.4455245760757779No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA235980.4208257193385985No Hit
GTTGGGTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGATAT233600.4165814392639063No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCATAATATAG197000.35131225828334567No Hit
ATATTATGCTTGGCTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT195240.3481736310012203No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCATAATAT173070.3086376271121758No Hit
CCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAG115890.20666790666221793No Hit
CTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACGTTCTCCTGA114580.20433176931018143No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA107110.19101043647070634No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT94680.16884388129069627No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT93590.16690007234892548No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT90250.16094381375671038No Hit
TTGCTATATTATGCTTGGCTATAATTTTTCATCTTTCCCTTGCGGTACTA81470.14528634356519882No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC80960.14437685497776478No Hit
GGGTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGATATTGG72090.12855888680023547No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT70880.1264010805437743No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA68060.12137214364855077No Hit
ATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGC67720.12076581792359475No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC67130.11971366445970048No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAAT66490.11857234544801853No Hit
GGGTTAGTCCTTGCTATATTATGCTTGGCTATAATTTTTCATCTTTCCCT65460.11673553516359293No Hit
GTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCT64850.11564771548058358No Hit
GTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATT63530.11329374501898959No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT62960.11227725777421037No Hit
GTGTTGGGTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGAT62120.11077927657137782No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGAGTGATAT62090.11072577724270524No Hit
GTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTACCGCAAG62010.11058311236624499No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAG61090.10894246628695221No Hit
CCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAAT60350.10762281617969498No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCT59170.1055185092519064No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG58690.10466251999314495No Hit
GTGTATGAACATGAGGGTGTTTTCTCGTGTGAATGAGGGTTTTATGTTGT58370.104091860487304No Hit
ATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCATAAT57320.1022193839837633No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC56170.10016857638464732No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)