FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922226

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922226
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4634024
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCTGTACTGTGTCAAATCGTTAAGAGCGAAGGAGGGACGCAGGAGGTG185490.4002784620882412No Hit
GTACAGGAATCTCTACCTGTTATCCATCGACTACGCCTCTCGGCCTCGCC150460.32468541380018745No Hit
TGGTAGGAGAGCGTTCTAAGAGCGGAGAAGTTGAATCGAGAGGATCAATG68930.14874761114746063No Hit
GTATCAACGCAGAGTACGGGTATGTCATCTCTGGCTAGAAGCTTTTCTTG63490.13700835386264723No Hit
GTTTTAAAGAGCACGTGGTAGGAGAGCGTTCTAAGAGCGGAGAAGTTGAA51700.11156610323986237No Hit
TTTCTTGGCAGTGTGATTACCACACCTTCGCTACTTTTAATTCACTCCTC48150.10390537468083895No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA259850.075.3174741
GGTATCA95750.072.2140961
TATCAAC352900.055.2391322
ATCAACG371450.052.3203243
CAACGCA377700.051.2502985
TCAACGC383150.050.598414
AACGCAG384550.050.446246
ACGCAGA390200.049.440227
CGCAGAG390350.049.3907288
GCAGAGT414000.046.38099
AGAGTAC405200.036.32232710-11
CAGAGTA413250.031.54719210-11
AGTACGG483400.030.02582212-13
GTACAGG83650.030.019341
GTACTGT86250.029.7359716
TACAGGA84700.029.7174532
GAATCTC79650.029.435077
CCTGTAC87350.028.8158933
TACTGTG91700.028.0978577
TCCTGTA90750.027.6707172
Produced by FastQC (version 0.11.5)