FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922244

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922244
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6004511
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT309170.5148962171940396No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138250.23024356188205836No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129710.2160209216037742No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC88580.14752242105976657No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA339900.075.188031
GGTATCA144150.069.745491
TATCAAC490350.052.6336362
ATCAACG511200.050.4520383
AACGCAG519300.049.801556
TCAACGC519200.049.6403274
CAACGCA526350.048.9434285
ACGCAGA537250.048.037317
CGCAGAG549100.047.0102738
GCAGAGT572200.045.1124469
AGAGTAC556650.034.8888910-11
CAGAGTA564850.031.71616410-11
AGTACGG475700.029.57976212-13
GAGTACT179200.028.12990812-13
GAGTACG477550.026.91488512-13
AGTACTT203050.026.06928612-13
TACGGGT122200.025.13582814-15
TACGGGC124450.024.87252614-15
GTACTTT213600.023.60960414-15
GTACGGG520100.023.53737814-15
Produced by FastQC (version 0.11.5)