FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922245

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922245
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6004511
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC103630.17258691007477545No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT61080.10172352086622874No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA263950.074.613051
GGTATCA118700.061.854731
TATCAAC392550.050.047192
AACGCAG405500.048.2335786
ATCAACG405900.048.1955263
TCAACGC411550.047.533474
ACGCAGA413400.047.3305747
CAACGCA416900.046.889075
CGCAGAG424950.046.028998
GCAGAGT446500.043.6508879
AGAGTAC432750.034.37407310-11
CAGAGTA440550.030.2012710-11
AGTACGG515000.028.75551412-13
TACGGGT122450.025.91514214-15
GAGTACG518400.025.57828912-13
TACGGGC143750.024.95096814-15
TACGGGA178050.023.85251414-15
GTACGGG548350.023.22398614-15
TACGGGG158200.020.52882614-15
ACGGGTA30050.020.49080516-17
Produced by FastQC (version 0.11.5)