FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922262

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922262
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7534153
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA525290.6972117502790294No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC438560.5820959569045121No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA386160.5125460021849836No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG383770.5093737809678142No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA338730.44959267485011256No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC334930.44454897584373454No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC315250.41842792414754515No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC306790.40719905741229306No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT292660.38844446084384004No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC288390.38277693590772577No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA255520.33914893950255587No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC232560.308674379190335No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA230370.3057676158156066No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC220930.29323800565239383No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT214320.2844646239597205No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA188910.250738205077598No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG183760.24390266563474355No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA180460.23952261123446789No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC166950.2215909339775818No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA159910.21224681792366046No Hit
CTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGC152770.2027699729485186No Hit
ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT150520.1997835722210579No Hit
GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA147810.19618661845598306No Hit
GTAATAGCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCA144290.19151456042902235No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC143240.19012090675620735No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC142440.18905907538644356No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA126580.16800826848087635No Hit
TGAAATACAGAGAAAAAGGCATCCCAAAAAAAAAAAAAAAAAAAAAAAAA125120.16607042623105744No Hit
AAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAA123200.16352203094362433No Hit
GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG121880.16177000918351406No Hit
ATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGA119870.15910215786698254No Hit
CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA119320.15837214880026992No Hit
ATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCA118010.1566333999322817No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC116600.15476192214307302No Hit
GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA114990.1526249865114234No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG114910.15251880337444698No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC114480.15194806901319896No Hit
CCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCT106830.1417943065398327No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA103700.13763989130563184No Hit
ATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGT103630.1375469810607775No Hit
TGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATG100450.1333262013659664No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT100400.13325983690535617No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT99910.13260946519137584No Hit
AACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTA98590.1308574434312656No Hit
GGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGTTTCCCTTCGG96250.1277515866747065No Hit
TGAAATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTAC95830.1271941252055805No Hit
GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG95250.12642429746250175No Hit
GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG94610.12557483236669073No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC92930.12334498649018676No Hit
AATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTA92780.12314589310835605No Hit
ATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGT92210.12238933825739932No Hit
ATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACT89010.11814201277834417No Hit
ACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTC88610.11761109709346226No Hit
GTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAA86010.11416014514172992No Hit
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG85320.11324431558530866No Hit
TCTTTAATCCTCTCACTCCACAAATTCATAAGCTTCACCATTTTACTTCA85250.11315140534045433No Hit
TAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGG84080.11159847696217479No Hit
GCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAG82590.10962081603598972No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT82410.10938190397779285No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT82270.10919608348808418No Hit
GCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGT82240.10915626481171806No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80480.10682023579823771No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT80140.1063689574660881No Hit
GTATTTGCATTATTGATGGTTTTTACTATGTTGGGTATGCTGTTCGATTT80130.10635568457396603No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT77250.10253309164281639No Hit
GGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCT77200.10246672718220616No Hit
TCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTA76050.10094034458817068No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGAG52550.081.7883766
AATACAG70750.062.4133834
GTATCAA313050.062.313091
ATACAGA78550.055.0889365
AAAGGTC26400.053.722017
ACAGAGA79600.053.2711687
GGTATCA195700.052.246131
AAGGTCA27500.050.7877548
TCTCTGC74400.049.5435378
CTCTGCA76300.048.8759549
TTCTCTG77200.048.2128947
ATCAACG429350.045.1319923
TGACAGA16450.045.0776527
AACGCAG431350.045.0633966
TATCAAC436950.044.6290322
CAACGCA437250.044.311865
AGGTCAA30700.044.086899
TCAACGC439900.043.9186024
CGCAGAG444200.043.6949588
ACGCAGA447450.043.3775837
Produced by FastQC (version 0.11.5)