FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922279

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922279
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4809671
Sequences flagged as poor quality0
Sequence length150
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101520.21107472839618346No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT57490.11953000527478908No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA57380.11930129940280738No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56980.11846964168651036No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA50140.10424829473783133No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48320.10046425212867992No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA317100.059.661161
CAACGCA437400.043.813755
GGTATCA217350.041.8122671
ATCAACG456000.041.6955073
TCAACGC457750.041.5356674
TATCAAC460250.041.4977572
AACGCAG468850.040.9208346
CGCAGAG471950.040.455438
ACGCAGA477400.040.1293077
TAAGACT29100.039.0897879
GCAGAGT488450.038.9414339
ATTTAGG33150.034.048421
GTACATG38350.032.279911
TACATGC36000.031.996222
ATGCTAA37800.029.9012285
TAGGCAT20550.026.2742655
TTTAGGG44100.024.3236562
TACGGGT100750.023.5088834
ATGTACT37550.023.1983224
GTGTATG44350.022.9870471
Produced by FastQC (version 0.11.5)