FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004922290

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922290
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1123126
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36190.3222256452081067No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT33160.2952473720668919No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC31540.2808233448428761No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT28460.2533998856762287No Hit
TGAAATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAA27320.24324964429636567No Hit
TCATAAAAAGGTCAATCCAACCACCCAAATCAAGCGTTACGTACTGCAGT25190.22428471961293744No Hit
GGTCTATACCCTGCAACTTAACGCCCAGTTAAGACTCGGTTTCCCTTCGG24110.21466870146359357No Hit
TGAAATACAGAGAAAAAGGCATCCCAAAACAACCCCACGTACTGCAGTCA21970.19561473957507886No Hit
TCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT20340.1811016751459765No Hit
GTATACGTCCACTTTCGTGTTTGCACAGTGCTGTGTTTTTAATAAACAGT18060.16080119238625054No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17730.15786296461839544No Hit
TCATAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAA17140.15260976951829092No Hit
CGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGC16680.14851405808431112No Hit
TGAAATACAGAGAAAAAGGCCTGTCTCTTATACACATCTCCGAGCCCACG16080.14317182577912008No Hit
TGAAATACAGAGAAAAAGGCATCCCAAAACAACCCCCCGTACTCTGCGTT15710.1398774491909189No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15060.13409003086029528No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACCGGGGCTTCGATCAAG14310.12741224047880648No Hit
ATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTC13730.12224808258378846No Hit
TCAGGTAACAAGCTAAATGAAACCACCAGAAACCAGGAACGTACTGCAGT12650.11263206443444457No Hit
TCATGTAACAAGCAAAATGAAACCACCAGAAACCAGGAACGTACTGCAGT12560.11183072958866591No Hit
CTTTATGAGGTCCGCTTGCTCTCGCGAGGTCGCTTCTCTTTGTATGCGCC12170.10835827859029175No Hit
GTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATGCAGTGT11830.10533101361735016No Hit
CTTCAAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTC11680.10399545554105238No Hit
ACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGATGCAGTGTACCCGC11250.10016685572233214No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)