FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922309

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922309
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9977360
Sequences flagged as poor quality0
Sequence length150
%GC38

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT121740.1220162447781778No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCA55500.048.1280365
TCAACGC54550.048.041344
AACGCAG57350.045.8227356
CGCAGAG56750.045.6726268
GTACGGG47850.043.18491
ATCAACG62750.041.8779533
ACGCAGA64050.041.1416137
GAGTACG37750.037.322051
TACGGGA23350.036.691212
GTCTCGC8200.034.3635941
AGTACGG40850.034.1909872
TACGGGG27050.032.737072
TAGGGGT67200.032.6767654
GGGGTTA68150.032.433526
GGGTTAG74550.029.455857
ACGGGAC7700.028.9852663
ATAGGGG73950.028.915093
TCTATAC112200.028.5544283
AGGGGTT71300.028.4758555
GTATCAA109750.027.715671
Produced by FastQC (version 0.11.5)