FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004922321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4011730
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT251450.6267869472771099No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG187660.4677782403102901No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT154460.3850209261341117No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT150260.37455162735278796No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA111270.27736163699949895No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT62770.15646616297706972No Hit
GTTATTTCTAGTTAATTCATTATGCAGAAGGTATAGGGGTTAGTCCTTGC61790.15402332659476087No Hit
TTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTA58640.14617135250876803No Hit
CCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAAT55440.13819474391347375No Hit
GTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATT54570.1360261034516281No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT54490.13582668823674574No Hit
ATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAAT54030.13468005075117218No Hit
GTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTACCGCAAG53250.13273575240606922No Hit
GCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATA52320.13041755053306178No Hit
CCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAG47920.11944971371453214No Hit
TACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTG42390.10566513698578918No Hit
GGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTT42010.10471791471509799No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG41270.10287332397743616No Hit
ACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATTTG40440.1008043911230317No Hit
GGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCT40280.10040556069326699No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)