FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922322

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922322
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5155847
Sequences flagged as poor quality0
Sequence length125
%GC49

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT214980.41696349794708804No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGACGCCTGC81130.15735532881406294No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55980.1085757587453623No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT53310.1033971721814088No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51980.10081757662707991No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA252650.079.344671
CGTACGG17200.071.682372
GCGTACG19000.066.071251
GCTATCA38900.065.118891
TATCAAC362850.055.26262
ATCAACG379750.054.823473
TCAACGC386800.053.9396134
AACGCAG388500.053.833416
CAACGCA394750.052.9384845
ACGCAGA413350.050.63777
CGCAGAG419100.049.9429478
GCAGAGT441800.047.186589
GGTATCA86550.046.4917071
CTATCAA64950.039.001152
GCGTATG7500.035.1200181
AGAGTAC428150.032.1530410-11
CAGAGTA439250.031.104510-11
GAGTACT128750.027.1387912-13
AGTACGG399500.026.02827812-13
GCATCAA35350.025.8414151
Produced by FastQC (version 0.11.5)